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Malaria Ag specific T cells induced by Plasmodium falciparum (Pf) Ags play a critical role in the control of malarial parasites. However, the precise mechanisms of their induction are not fully understood. In this study we examined the influence of Pf Ag on human peripheral blood mononuclear cell proliferation by examining the expression of IL-2 receptor (IL-2R), IL-2 production, and IL-2 signaling. Pf Ag specific CD4+ and CD8+ T cells preferentially expressed the low affinity IL-2R CD25, and expressed high levels of IL-2 mRNA. Furthermore, a quantitative analysis of IL-2 production in Pf Ag stimulated PBMCs revealed that the levels of IL-2 were much higher in PBMCs stimulated with whole Pf Ag than in non-Pf Ag stimulated PBMCs. This effect was particularly prominent in Pf Ag stimulated CD4+ T cells, in which Pf Ag stimulated CD4+ T cells produced around 10 fold greater amounts of IL-2 compared to non-Pf Ag stimulated CD4+ T cells. Finally, IL-2 signaling was investigated using cytokine secretion and STAT-5 phosphorylation assay. We found that the IL-2 production of CD4+ and CD8+ T cells was increased after stimulation with Pf Ag. We also showed that CD4+ T cells stimulated with Pf Ag produced only IL-2. In addition, IL-2 significantly induced the phosphorylation of STAT-5 in both CD4+ and CD8+ T cells stimulated with Pf Ag. Furthermore, IL-2 secretion by CD4+ T cells was significantly inhibited by a neutralizing anti-CD25 Ab. Taken together, these data indicate that



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I. Field of the Invention
The present invention relates generally to modular building systems of the type having a plurality of panels arranged in end-to-end abutting relationship to form an elongated building or structure and, more particularly, to such a building system that is readily movable between a disassembled and a erected state.
II. Related Art
There are a variety of different types of prefabricated building systems known in the art. As such, these various systems are designed to provide a versatile alternative for the design and construction of various buildings.
One type of building system utilizes wood structural members, and may be prefabricated and shipped in a disassembled state to an intended building site. The structural members are then arranged into the final building configuration. To perform the shipping and erection steps, the known wooden building systems typically require a number of separate structural assemblies and components, which must be carefully handled, often requiring multiple workers.
Modular building systems also exist which are formed from a plurality of generally parallel, corrugated metal building members, which may be prefabricated, shipped, and erected as a single structure. For example, U.S. Pat. No. 4,647,047 describes a modular building having a plurality of building segments, which may be constructed in a longitudinally-extending arrangement, and joined together, end-to-end, to form the structure. However, the building systems described in the ‘047 patent have numerous disadvantages. For example, a substantial amount of care and skill is required to assemble the known metal building systems in the manner taught in the ‘047 patent. In addition, the manufacture of the known metal building systems is relatively expensive. Moreover, the known metal building systems require many welds or other sealing techniques, and are thus also expensive to construct and to maintain.

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